celescope 1.4.0

Single Cell Analysis Pipelines

CeleScope

CeleScope is a collection of bioinfomatics analysis pipelines developed at Singleron to process single cell sequencing data generated with Singleron products. These pipelines take paired-end FASTQ files as input and generate output files which can be used for downstream data analysis as well as a summary of QC criteria.

Detailed docs can be found in manual.

Hardware/Software Requirements

• minimum 32GB RAM(to run STAR aligner)
• conda
• git

Installation

1. Clone repo

git clone https://gitee.com/singleron-rd/celescope.git
# or 
git clone https://github.com/singleron-RD/CeleScope.git

2. Install conda packages

cd CeleScope
conda create -n celescope
conda activate celescope
conda install -y --file conda_pkgs.txt --channel conda-forge --channel bioconda --channel r --channel imperial-college-research-computing

3. Install celescope

pip install celescope
# Use pypi mirror to accelerate downloading if you are in china
pip install -i https://pypi.mirrors.ustc.edu.cn/simple/ celescope

Reference genome

Homo sapiens

mkdir hs_ensembl_99
cd hs_ensembl_99

wget ftp://ftp.ensembl.org/pub/release-99/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
wget ftp://ftp.ensembl.org/pub/release-99/gtf/homo_sapiens/Homo_sapiens.GRCh38.99.gtf.gz

gunzip Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz
gunzip Homo_sapiens.GRCh38.99.gtf.gz

conda activate celescope
celescope rna mkref \
 --genome_name Homo_sapiens_ensembl_99 \
 --fasta Homo_sapiens.GRCh38.dna.primary_assembly.fa \
 --gtf Homo_sapiens.GRCh38.99.gtf

Mus musculus

mkdir mmu_ensembl_99
cd mmu_ensembl_99

wget ftp://ftp.ensembl.org/pub/release-99/fasta/mus_musculus/dna/Mus_musculus.GRCm38.dna.primary_assembly.fa.gz
wget ftp://ftp.ensembl.org/pub/release-99/gtf/mus_musculus/Mus_musculus.GRCm38.99.gtf.gz

gunzip Mus_musculus.GRCm38.dna.primary_assembly.fa.gz 
gunzip Mus_musculus.GRCm38.99.gtf.gz

conda activate celescope
celescope rna mkref \
 --genome_name Mus_musculus_ensembl_99 \
 --fasta Mus_musculus.GRCm38.dna.primary_assembly.fa \
 --gtf Mus_musculus.GRCm38.99.gtf

Quick start